ada-2;daf-16;daf-2

Lifespan changes: From wild type to ada-2;daf-16;daf-2

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Genetic mutants with ada-2, daf-16, daf-2 alterations

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    25

  • Lifespan (days)

    8.6

  • Lifespan change (compared to wild type)

    -41.50%

  • Phenotype

    Fifty-seven gene inactivations (corresponding to 55 RNAi clones) more dramatically shortened the life span of daf-2 animals compared with daf-2;daf-16, but still shortened the life span of daf-2;daf-16 animals, suggesting that they function in a parallel/converging pathway to insulin/IGF1 signaling

  • Lifespan comparisons

    Triple mutant ada-2(RNAi);daf-16(mgDf47);daf-2(e1370) has a lifespan of 8.6 days, while double mutant daf-16(mgDf47);daf-2(e1370) has a lifespan of 10.1 days, single mutant ada-2(RNAi) has a lifespan of 11.5 days and wild type has a lifespan of 14.7 days.

  • Type of interaction
    See methods

    Contains dependence

  • Citation
    View abstract

    Samuelson AV et al., 2007, Gene activities that mediate increased life span of C. elegans insulin-like signaling mutants. Genes Dev. 21(22):2976-94 PubMed 18006689 Click here to select all mutants from this PubMed ID in the graph

Search genes: ada-2 daf-16 daf-2 ada-2;daf-16;daf-2
  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

ADA (histone acetyltransferase complex) subunit


Locus: CELE_F32A5.1


Wormbase description: none available


  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Forkhead box protein O;hypothetical protein


Locus: CELE_R13H8.1


Wormbase description: daf-16 encodes the sole C. elegans forkhead box O (FOXO) homologue; DAF-16 functions as a transcription factor that acts in the insulin/IGF-1-mediated signaling (IIS) pathway that regulates dauer formation, longevity, fat metabolism, stress response, and innate immunity; DAF-16 regulates these various processes through isoform-specific expression, isoform-specific regulation by different AKT kinases, and differential regulation of target genes; DAF-16 can interact with the CBP-1 transcription cofactor in vitro, and interacts genetically with other genes in the insulin signaling and with daf-12, which encodes a nuclear hormone receptor; DAF-16 is activated in response to DNA damage during development and co-regulated by EGL-27, alleviates DNA-damage-induced developmental arrest by inducing DAF-16-associated element (DAE)-regulated genes; DAF-16 is broadly expressed but displays isoform-specific tissue enrichment; DAF-16 localizes to both the cytoplasm and the nucleus, with the ratio between the two an important regulator of function.


  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Insulin-like receptor subunit beta;Receptor protein-tyrosine kinase;hypothetical protein


Locus: CELE_Y55D5A.5


Wormbase description: daf-2 encodes a receptor tyrosine kinase that is the C. elegans insulin/IGF receptor ortholog; DAF-2 activity is required for a number of processes in C. elegans, including embryonic and larval development, formation of the developmentally arrested dauer larval stage (diapause), larval developmental timing, adult longevity, reproduction, fat storage, salt chemotaxis learning, and stress resistance, including response to high temperature, oxidative stress, and bacterial infection; DAF-2 signals through a conserved PI 3-kinase pathway to negatively regulate the activity of DAF-16, a Forkhead-related transcription factor, by inducing its phosphorylation and nuclear exclusion; in addition, DAF-2 negatively regulates the nuclear localization, and hence transcriptional activity, of SKN-1 in intestinal nuclei; amongst the 38 predicted insulin-like molecules in C. elegans, genetic and microarray analyses suggest that at least DAF-28, INS-1, and INS-7 are likely DAF-2 ligands; genetic mosaic and tissue-specific promoter studies indicate that daf-2 can function cell nonautonomously and within multiple cell types to influence dauer formation and adult lifespan, likely by regulating the production of secondary endocrine signals that coordinate growth and longevity throughout the animal; temporal analysis of daf-2 function indicates that daf-2 regulates lifespan, reproduction, and diapause independently, at distinct times during the animal's life cycle.


Orthologs of ada-2;daf-16;daf-2 in SynergyAge
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Species Gene
Orthologs of ada-2 in SynergyAge
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Species Gene
Orthologs of daf-16 in SynergyAge
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Species Gene
Orthologs of daf-2 in SynergyAge
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Species Gene
Drosophila melanogaster InR
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SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.

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