aak-2;daf-2

Lifespan changes: From wild type to aak-2;daf-2 / From aak-2;daf-2 to multiple mutants

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Genetic mutants with aak-2, daf-2 alterations

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    25

  • Lifespan (days)

    24.5

  • Lifespan change (compared to wild type)

    66.67%

  • Phenotype

    Forty-one gene inactivations functioned specifically within the daf-2 pathway to shorten life span, not decreasing the life span of daf-2;daf-16 animals

  • Lifespan comparisons

    Double mutant aak-2(RNAi);daf-2(e1370) has a lifespan of 24.5 days, while single mutant daf-2(e1370) has a lifespan of 36.7 days, single mutant aak-2(RNAi) has a lifespan of 6.8 days and wild type has a lifespan of 14.7 days.

  • Type of interaction
    See methods

    Opposite lifespan effects of single mutants

  • Citation
    View abstract

    Samuelson AV et al., 2007, Gene activities that mediate increased life span of C. elegans insulin-like signaling mutants. Genes Dev. 21(22):2976-94 PubMed 18006689 Click here to select all mutants from this PubMed ID in the graph

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    25

  • Diet

    NGM

  • Lifespan (days)

    23.3

  • Lifespan change (compared to wild type)

    14.78%

  • Lifespan comparisons

    Double mutant aak-2(ok524);daf-2(RNAi) has a lifespan of 23.3 days, while single mutant daf-2(RNAi) has a lifespan of 30.6 days, single mutant aak-2(ok524) has a lifespan of 17.1 days and wild type has a lifespan of 20.3 days.

  • Type of interaction
    See methods

    Opposite lifespan effects of single mutants

  • Citation
    View abstract

    Rogers AN et al., 2011, Life span extension via eIF4G inhibition is mediated by posttranscriptional remodeling of stress response gene expression in C. elegans. Cell Metab. 14(1):55-66 PubMed 21723504 Click here to select all mutants from this PubMed ID in the graph

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    20

  • Lifespan (days)

    25.1

  • Lifespan change (compared to wild type)

    25.50%

  • Phenotype

    Double mutants of aak-2(ok524) and stronger daf-2 reduction-of-function alleles have shortened lifespans compared with daf-2 single mutants, although they live longer than aak-2(ok524) single mutants. Therefore, aak-2-independent as well as aak-2-dependent mechanisms are required to produce the exceptionally long lifespans of daf-2 mutants.

  • Lifespan comparisons

    Double mutant aak-2(ok524);daf-2(e1368) has a lifespan of 25.1 days, while single mutant aak-2(ok524) has a lifespan of 17.6 days, single mutant daf-2(e1368) has a lifespan of 36.7 days and wild type has a lifespan of 20.0 days.

  • Type of interaction
    See methods

    Opposite lifespan effects of single mutants

  • Citation
    View abstract

    Apfeld J et al., 2004, The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans. Genes Dev. 18(24):3004-9 PubMed 15574588 Click here to select all mutants from this PubMed ID in the graph

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    25

  • Lifespan (days)

    22.5

  • Lifespan change (compared to wild type)

    75.78%

  • Lifespan comparisons

    Double mutant aak-2(ok524);daf-2(e1370) has a lifespan of 22.5 days, while single mutant daf-2(e1370) has a lifespan of 31.8 days, single mutant aak-2(ok524) has a lifespan of 10.2 days and wild type has a lifespan of 12.8 days.

  • Type of interaction
    See methods

    Opposite lifespan effects of single mutants

  • Citation
    View abstract

    Chen D et al., 2013, Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans. Cell Rep. 5(6):1600-10 PubMed 24332851 Click here to select all mutants from this PubMed ID in the graph

  • Temperature °C

    25

  • Lifespan (days)

    18.6

  • Lifespan change (compared to wild type)

    43.08%

  • Lifespan comparisons

    Double mutant aak-2(ok524);daf-2(e1370) has a lifespan of 18.6 days, while single mutant daf-2(e1370) has a lifespan of 33.7 days, single mutant aak-2(ok524) has a lifespan of 11.3 days and wild type has a lifespan of 13.0 days.

  • Type of interaction
    See methods

    Opposite lifespan effects of single mutants

  • Citation
    View abstract

    Chen D et al., 2013, Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans. Cell Rep. 5(6):1600-10 PubMed 24332851 Click here to select all mutants from this PubMed ID in the graph

  • Temperature °C

    20

  • Lifespan (days)

    29.4

  • Lifespan change (compared to wild type)

    47.00%

  • Phenotype

    Double mutants of aak-2(ok524) and stronger daf-2 reduction-of-function alleles have shortened lifespans compared with daf-2 single mutants, although they live longer than aak-2(ok524) single mutants. Therefore, aak-2-independent as well as aak-2-dependent mechanisms are required to produce the exceptionally long lifespans of daf-2 mutants.

  • Lifespan comparisons

    Double mutant aak-2(ok524);daf-2(e1370) has a lifespan of 29.4 days, while single mutant aak-2(ok524) has a lifespan of 17.6 days, single mutant daf-2(e1370) has a lifespan of 43.2 days and wild type has a lifespan of 20.0 days.

  • Type of interaction
    See methods

    Opposite lifespan effects of single mutants

  • Citation
    View abstract

    Apfeld J et al., 2004, The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans. Genes Dev. 18(24):3004-9 PubMed 15574588 Click here to select all mutants from this PubMed ID in the graph

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    20

  • Lifespan (days)

    17.2

  • Lifespan change (compared to wild type)

    -14.00%

  • Phenotype

    daf-2(m577); aak-2(ok524) double mutants have lifespans that are indistinguishable from those of aak-2(ok524) single mutants

  • Lifespan comparisons

    Double mutant aak-2(ok524);daf-2(m577) has a lifespan of 17.2 days, while single mutant aak-2(ok524) has a lifespan of 17.6 days, single mutant daf-2(m577) has a lifespan of 24.2 days and wild type has a lifespan of 20.0 days.

  • Type of interaction
    See methods

    Opposite lifespan effects of single mutants

  • Citation
    View abstract

    Apfeld J et al., 2004, The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans. Genes Dev. 18(24):3004-9 PubMed 15574588 Click here to select all mutants from this PubMed ID in the graph

Search genes: aak-2 daf-2
  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

5'-AMP-activated protein kinase catalytic subunit alpha-2


Locus: CELE_T01C8.1


Wormbase description: aak-2 encodes one of two C. elegans homologs of the catalytic alpha subunit of AMP-activated protein kinases (AMPKs); in C. elegans, aak-2 functions downstream of environmental stressors, energy level signals (AMP:ATP ratio), and daf-2-mediated insulin signaling to positively regulate adult lifespan; in regulating lifespan, aak-2 likely acts in parallel with daf-16/FOXO; aak-2 activity is also required for dauer formation in daf-2 mutant animals at high temperature in a manner independent of the AMP:ATP ratio; in the germline, aak-2 functions downstream of daf-2 and daf-7, and in parallel to par-4 and aak-1, to negatively regulate germline proliferation during dauer development; in vitro, AAK-2 exhibits AMP-enhanced kinase activity against a known AMPK substrate, the SAMS peptide.


  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Insulin-like receptor subunit beta;Receptor protein-tyrosine kinase;hypothetical protein


Locus: CELE_Y55D5A.5


Wormbase description: daf-2 encodes a receptor tyrosine kinase that is the C. elegans insulin/IGF receptor ortholog; DAF-2 activity is required for a number of processes in C. elegans, including embryonic and larval development, formation of the developmentally arrested dauer larval stage (diapause), larval developmental timing, adult longevity, reproduction, fat storage, salt chemotaxis learning, and stress resistance, including response to high temperature, oxidative stress, and bacterial infection; DAF-2 signals through a conserved PI 3-kinase pathway to negatively regulate the activity of DAF-16, a Forkhead-related transcription factor, by inducing its phosphorylation and nuclear exclusion; in addition, DAF-2 negatively regulates the nuclear localization, and hence transcriptional activity, of SKN-1 in intestinal nuclei; amongst the 38 predicted insulin-like molecules in C. elegans, genetic and microarray analyses suggest that at least DAF-28, INS-1, and INS-7 are likely DAF-2 ligands; genetic mosaic and tissue-specific promoter studies indicate that daf-2 can function cell nonautonomously and within multiple cell types to influence dauer formation and adult lifespan, likely by regulating the production of secondary endocrine signals that coordinate growth and longevity throughout the animal; temporal analysis of daf-2 function indicates that daf-2 regulates lifespan, reproduction, and diapause independently, at distinct times during the animal's life cycle.


Orthologs of aak-2;daf-2 in SynergyAge
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Species Gene
Orthologs of aak-2 in SynergyAge
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Species Gene
Orthologs of daf-2 in SynergyAge
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Species Gene
Drosophila melanogaster InR
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SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.

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