aak-2;daf-16

Lifespan changes: From wild type to aak-2;daf-16

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Genetic mutants with aak-2, daf-16 alterations

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    25

  • Diet

    NGM

  • Lifespan (days)

    11.3

  • Lifespan change (compared to wild type)

    -44.33%

  • Lifespan comparisons

    Double mutant aak-2(ok524);daf-16(RNAi) has a lifespan of 11.3 days, while single mutant daf-16(RNAi) has a lifespan of 12.8 days, single mutant aak-2(ok524) has a lifespan of 17.1 days and wild type has a lifespan of 20.3 days.

  • Type of interaction
    See methods

    Almost additive (negative)

  • Citation
    View abstract

    Rogers AN et al., 2011, Life span extension via eIF4G inhibition is mediated by posttranscriptional remodeling of stress response gene expression in C. elegans. Cell Metab. 14(1):55-66 PubMed 21723504 Click here to select all mutants from this PubMed ID in the graph

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    20

  • Lifespan (days)

    14.6

  • Lifespan change (compared to wild type)

    -27.00%

  • Phenotype

    daf-16(mu86); aak-2(ok524) double mutants have 15% shorter lifespans than either single mutant. Therefore, aak-2 is able to influence lifespan in a daf-16-independent manner.

  • Lifespan comparisons

    Double mutant aak-2(ok524);daf-16(mu86) has a lifespan of 14.6 days, while single mutant aak-2(ok524) has a lifespan of 17.6 days, single mutant daf-16(mu86) has a lifespan of 17.1 days and wild type has a lifespan of 20.0 days.

  • Type of interaction
    See methods

    Synergistic (negative)

  • Citation
    View abstract

    Apfeld J et al., 2004, The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans. Genes Dev. 18(24):3004-9 PubMed 15574588 Click here to select all mutants from this PubMed ID in the graph

Search genes: aak-2 daf-16
  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

5'-AMP-activated protein kinase catalytic subunit alpha-2


Locus: CELE_T01C8.1


Wormbase description: aak-2 encodes one of two C. elegans homologs of the catalytic alpha subunit of AMP-activated protein kinases (AMPKs); in C. elegans, aak-2 functions downstream of environmental stressors, energy level signals (AMP:ATP ratio), and daf-2-mediated insulin signaling to positively regulate adult lifespan; in regulating lifespan, aak-2 likely acts in parallel with daf-16/FOXO; aak-2 activity is also required for dauer formation in daf-2 mutant animals at high temperature in a manner independent of the AMP:ATP ratio; in the germline, aak-2 functions downstream of daf-2 and daf-7, and in parallel to par-4 and aak-1, to negatively regulate germline proliferation during dauer development; in vitro, AAK-2 exhibits AMP-enhanced kinase activity against a known AMPK substrate, the SAMS peptide.


  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Forkhead box protein O;hypothetical protein


Locus: CELE_R13H8.1


Wormbase description: daf-16 encodes the sole C. elegans forkhead box O (FOXO) homologue; DAF-16 functions as a transcription factor that acts in the insulin/IGF-1-mediated signaling (IIS) pathway that regulates dauer formation, longevity, fat metabolism, stress response, and innate immunity; DAF-16 regulates these various processes through isoform-specific expression, isoform-specific regulation by different AKT kinases, and differential regulation of target genes; DAF-16 can interact with the CBP-1 transcription cofactor in vitro, and interacts genetically with other genes in the insulin signaling and with daf-12, which encodes a nuclear hormone receptor; DAF-16 is activated in response to DNA damage during development and co-regulated by EGL-27, alleviates DNA-damage-induced developmental arrest by inducing DAF-16-associated element (DAE)-regulated genes; DAF-16 is broadly expressed but displays isoform-specific tissue enrichment; DAF-16 localizes to both the cytoplasm and the nucleus, with the ratio between the two an important regulator of function.


Orthologs of aak-2;daf-16 in SynergyAge
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Orthologs of aak-2 in SynergyAge
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Orthologs of daf-16 in SynergyAge
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SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.

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