aak-2;daf-2;rsks-1

Lifespan changes: From wild type to aak-2;daf-2;rsks-1

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Genetic mutants with aak-2, daf-2, rsks-1 alterations

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    20

  • Diet

    HT115

  • Lifespan (days)

    40.28

  • Lifespan change (compared to wild type)

    175.89%

  • Lifespan comparisons

    Triple mutant aak-2(OE);daf-2(e1370);rsks-1(ok1255) has a lifespan of 40.28 days, while single mutant aak-2(OE) has a lifespan of 21.98 days, double mutant daf-2(e1370);rsks-1(ok1255) has a lifespan of 50.51 days and wild type has a lifespan of 14.6 days.

  • Citation
    View abstract

    Hou L et al., 2016, A Systems Approach to Reverse Engineer Lifespan Extension by Dietary Restriction. Cell Metab. 23(3):529-40 PubMed 26959186 Click here to select all mutants from this PubMed ID in the graph

  • Temperature °C

    20

  • Diet

    HT115

  • Lifespan (days)

    36.99

  • Lifespan change (compared to wild type)

    75.31%

  • Lifespan comparisons

    Triple mutant aak-2(OE);daf-2(e1370);rsks-1(ok1255) has a lifespan of 36.99 days, while single mutant aak-2(OE) has a lifespan of 26.75 days, double mutant daf-2(e1370);rsks-1(ok1255) has a lifespan of 42.86 days and wild type has a lifespan of 21.1 days.

  • Citation
    View abstract

    Hou L et al., 2016, A Systems Approach to Reverse Engineer Lifespan Extension by Dietary Restriction. Cell Metab. 23(3):529-40 PubMed 26959186 Click here to select all mutants from this PubMed ID in the graph

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    25

  • Lifespan (days)

    22.1

  • Lifespan comparisons

    Triple mutant aak-2(RNAi);daf-2(e1370);rsks-1(ok1255) has a lifespan of 22.1 days, while double mutant daf-2(e1370);rsks-1(ok1255) has a lifespan of 50.1 days.

  • Citation
    View abstract

    Chen D et al., 2013, Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans. Cell Rep. 5(6):1600-10 PubMed 24332851 Click here to select all mutants from this PubMed ID in the graph

  • Temperature °C

    25

  • Lifespan (days)

    21.8

  • Lifespan comparisons

    Triple mutant aak-2(RNAi);daf-2(e1370);rsks-1(ok1255) has a lifespan of 21.8 days, while double mutant daf-2(e1370);rsks-1(ok1255) has a lifespan of 50.8 days.

  • Citation
    View abstract

    Chen D et al., 2013, Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans. Cell Rep. 5(6):1600-10 PubMed 24332851 Click here to select all mutants from this PubMed ID in the graph

  • Temperature °C

    25

  • Lifespan (days)

    20.0

  • Lifespan comparisons

    Triple mutant aak-2(RNAi);daf-2(e1370);rsks-1(ok1255) has a lifespan of 20.0 days, while double mutant daf-2(e1370);rsks-1(ok1255) has a lifespan of 51.3 days.

  • Citation
    View abstract

    Chen D et al., 2013, Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans. Cell Rep. 5(6):1600-10 PubMed 24332851 Click here to select all mutants from this PubMed ID in the graph

    Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.
  • Temperature °C

    25

  • Lifespan (days)

    26.6

  • Lifespan change (compared to wild type)

    107.81%

  • Phenotype

    A deletion in aak-2, which encodes the alpha catalytic subunit of AMPK suppressed the synergistic longevity of daf-2 and rsks-1

  • Lifespan comparisons

    Triple mutant aak-2(ok524);daf-2(e1370);rsks-1(ok1255) has a lifespan of 26.6 days, while double mutant daf-2(e1370);rsks-1(ok1255) has a lifespan of 58.7 days, single mutant aak-2(ok524) has a lifespan of 10.2 days and wild type has a lifespan of 12.8 days.

  • Type of interaction
    See methods

    Contains dependence

  • Citation
    View abstract

    Chen D et al., 2013, Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans. Cell Rep. 5(6):1600-10 PubMed 24332851 Click here to select all mutants from this PubMed ID in the graph

  • Temperature °C

    25

  • Lifespan (days)

    22.6

  • Lifespan change (compared to wild type)

    73.85%

  • Phenotype

    A deletion in aak-2, which encodes the alpha catalytic subunit of AMPK suppressed the synergistic longevity of daf-2 and rsks-1

  • Lifespan comparisons

    Triple mutant aak-2(ok524);daf-2(e1370);rsks-1(ok1255) has a lifespan of 22.6 days, while double mutant daf-2(e1370);rsks-1(ok1255) has a lifespan of 53.4 days, single mutant aak-2(ok524) has a lifespan of 11.3 days and wild type has a lifespan of 13.0 days.

  • Type of interaction
    See methods

    Contains dependence

  • Citation
    View abstract

    Chen D et al., 2013, Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans. Cell Rep. 5(6):1600-10 PubMed 24332851 Click here to select all mutants from this PubMed ID in the graph

Search genes: aak-2 daf-2 rsks-1 aak-2;daf-2;rsks-1
  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

5'-AMP-activated protein kinase catalytic subunit alpha-2


Locus: CELE_T01C8.1


Wormbase description: aak-2 encodes one of two C. elegans homologs of the catalytic alpha subunit of AMP-activated protein kinases (AMPKs); in C. elegans, aak-2 functions downstream of environmental stressors, energy level signals (AMP:ATP ratio), and daf-2-mediated insulin signaling to positively regulate adult lifespan; in regulating lifespan, aak-2 likely acts in parallel with daf-16/FOXO; aak-2 activity is also required for dauer formation in daf-2 mutant animals at high temperature in a manner independent of the AMP:ATP ratio; in the germline, aak-2 functions downstream of daf-2 and daf-7, and in parallel to par-4 and aak-1, to negatively regulate germline proliferation during dauer development; in vitro, AAK-2 exhibits AMP-enhanced kinase activity against a known AMPK substrate, the SAMS peptide.


  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Insulin-like receptor subunit beta;Receptor protein-tyrosine kinase;hypothetical protein


Locus: CELE_Y55D5A.5


Wormbase description: daf-2 encodes a receptor tyrosine kinase that is the C. elegans insulin/IGF receptor ortholog; DAF-2 activity is required for a number of processes in C. elegans, including embryonic and larval development, formation of the developmentally arrested dauer larval stage (diapause), larval developmental timing, adult longevity, reproduction, fat storage, salt chemotaxis learning, and stress resistance, including response to high temperature, oxidative stress, and bacterial infection; DAF-2 signals through a conserved PI 3-kinase pathway to negatively regulate the activity of DAF-16, a Forkhead-related transcription factor, by inducing its phosphorylation and nuclear exclusion; in addition, DAF-2 negatively regulates the nuclear localization, and hence transcriptional activity, of SKN-1 in intestinal nuclei; amongst the 38 predicted insulin-like molecules in C. elegans, genetic and microarray analyses suggest that at least DAF-28, INS-1, and INS-7 are likely DAF-2 ligands; genetic mosaic and tissue-specific promoter studies indicate that daf-2 can function cell nonautonomously and within multiple cell types to influence dauer formation and adult lifespan, likely by regulating the production of secondary endocrine signals that coordinate growth and longevity throughout the animal; temporal analysis of daf-2 function indicates that daf-2 regulates lifespan, reproduction, and diapause independently, at distinct times during the animal's life cycle.


  • Entrez ID
  • Symbol
  • GenAge
  • Wormbase ID

Ribosomal protein S6 kinase beta


Locus: CELE_Y47D3A.16


Wormbase description: rsks-1 encodes a putative ribosomal protein S6 kinase (S6K) required additively with IFG-1 for normally high levels of protein synthesis, and for normally short lifespan; RSKS-1's effect on lifespan is independent of DAF-16, ISP-1, and SIR-2.1, and does not correlate with juglone resistance, but does correlate with abnormally high resistance to starvation and (perhaps) thermotolerance; RSKS-1 is required for normal juglone resistance, as well as normally rapid growth and normal brood sizes; RSKS-1 is expressed in E-lineage embryonic cells, and in pharyngeal and hypodermal cells of larvae and adults; RSKS-1 is orthologous to human RPS6KB1 (OMIM:608938) and RPS6KB2 (OMIM:608939).


Orthologs of aak-2;daf-2;rsks-1 in SynergyAge
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Species Gene
Orthologs of aak-2 in SynergyAge
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Species Gene
Orthologs of daf-2 in SynergyAge
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Species Gene
Drosophila melanogaster InR
Orthologs of rsks-1 in SynergyAge
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About

SynergyAge database hosts high-quality, manually curated information about the synergistic and antagonistic lifespan effects of genetic interventions in model organisms, also allowing users to explore the longevity relationships between genes in a visual way.

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Computational Biology of Aging Group
Institute of Biochemistry, Ground floor
Splaiul Independentei 296, Bucharest, Romania

www.aging-research.group